Haruko Takeyama

Official Title

Professor
Department of Life Science and Medical Bioscience
School of Advanced Science and Engineering, Waseda University

Examples of Classes Taught

Current Topics in Bioscience

Research Fields

Marine biotechnology, Genome engineering, Single cell analysis

Research

 Marine biotechnology: Exploration of marine biological resources toward efficient use of biomaterials
 In the marine environment, bacteria in particular, are highly diverse and harbor unique and novel genes that are indispensable to research, pharmaceutics and industry. Our research has focused on marine bacteria for the environmental assessment and the exploitation of useful genes related to the production of biomaterials such as enzyme, drug, food, biodiesel, etc. In order to elucidate microbial flora in seawater, coral reef, marine sponge, etc. for the characterization of their function, the innovative omics technology capable of ultra-sensitive and high-throughput analysis is required. In our group, in addition to metagenomic research, we have begun including supporting technologies such as single-cell analysis technique, Raman microspectroscopy and microfluidics to assist us in optimizing throughput for searching of important or novel bacteria that would be of pharmaceutical or industrial value.

Selected Publications

1. Kogawa M, Hosokawa M, Nishikawa Y, Mori K, Takeyama H.
Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes.
Sci Rep. 2018 Feb 1. 8(1):2059. doi: 10.1038/s41598-018-20384-3.

2. Hosokawa M, Nishikawa Y, Kogawa M, Takeyama H.
Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics.
Sci Rep. 2017 Jul 12. 7(1):5199. doi: 10.1038/s41598-017-05436-4.

3. Yoda T, Hosokawa M, Takahashi K, Sakanashi C, Takeyama H, Kambara H.
Site-specific gene expression analysis using an automated tissue micro-dissection punching system.
Sci Rep. 2017 Jun 28. 7(1):4325. doi: 10.1038/s41598-017-04616-6.

4. Yoda T, Tanabe M, Tsuji T, Yoda T, Ishino S, Shirai T, Ishino Y, Takeyama H, Nishida H.
Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA.
Sci Rep. 2017 Mar 16. 7:44582. doi: 10.1038/srep44582.

5. Maruyama T, Mori T, Yamagishi K, Takeyama H.
SAG-QC: quality control of single amplified genome information by subtracting non-target sequences based on sequence compositions.
BMC Bioinformatics. 2017 Mar 4. 18(1):152. doi: 10.1186/s12859-017-1572-5.

Links

http://www.f.waseda.jp/haruko-takeyama/English/HomeEnglish.html